Analysis Note

ControlHaCaT keratinocytes cell lysate

Application

Anti-NHERF-2 Antibody, clone 32B6 is a highly specific mouse monoclonal antibody, that targets NHERF2 & has been tested in western blotting, ICC & IP.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in A431 and NHERF-2 transfected HT1080 cells. (Theisen, C. S., et al. (2007). Mole. Biol. Cell. 18:1220-1232).
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in Batt cell lysate. (Theisen, C. S., et al. (2007). Mole. Biol. Cell. 18:1220-1232).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Na(+)/H(+) exchange regulatory cofactor NHE-RF2 (NHERF-2) is also known as NHE3 kinase A regulatory protein E3KARP, SRY-interacting protein 1 (SIP-1), Sodium-hydrogen exchanger regulatory factor 2, Solute carrier family 9 isoform A3 regulatory factor 2 (SLC9A3R2), and Tyrosine kinase activator protein 1 (TKA-1). NHERF-2 is a Scaffold protein that links ezrin/moesin/radixin family members to the actin cytoskeleton and regulates their surface expression. NHERF-2 is critical for the cAMP-mediated phosphorylation and inhibition of SLC9A3. NHERF-2 is also thought to act as scaffold protein in the nucleus.

Immunogen

recombinant protein corresponding to human NHERF-2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HaCaT keratinocytes cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected NHERF-2 in 10 µg of HaCaT keratinocytes cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 37 kDa observed. An uncharacterized band may appear at ~41 kDa in some lysates.